Transmission of Mal de Río Cuarto virus by first and third-instar nymphs of Delphacodes kuscheli
Resumo
The ability of first and third-instar Delphacodes kuscheli Fennah (Hemiptera: Delphacidae) nymphs
to acquire and transmit Mal de Río Cuarto virus (MRCV), under controlled conditions, was investigated. First
and third-instar nymphs were allowed acquisition feeding separately on infected wheat plants for 48 hours. The
insects were then placed in groups of three for serial transmissions to healthy wheat plants, using inoculation
periods of 24 hours. Both instars of D. kuscheli were demonstrated to acquire and subsequently transmit the
virus. Nevertheless, transmission trials showed highest transmission efficiency and shortest latent period when
MRCV was acquired by first-instar nymphs.
to acquire and transmit Mal de Río Cuarto virus (MRCV), under controlled conditions, was investigated. First
and third-instar nymphs were allowed acquisition feeding separately on infected wheat plants for 48 hours. The
insects were then placed in groups of three for serial transmissions to healthy wheat plants, using inoculation
periods of 24 hours. Both instars of D. kuscheli were demonstrated to acquire and subsequently transmit the
virus. Nevertheless, transmission trials showed highest transmission efficiency and shortest latent period when
MRCV was acquired by first-instar nymphs.
Palavras-chave
Delphacidae; Reoviridae; experimental transmission; latent period
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